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THE NOVEL MOLECULAR IMAGING MARKERS OF 18F-FLUOROCHOLINE PET/CT WITH EXPRESSION OF CD47, miRNA-21 and miRNA-155 IN UNDERPINNING BREAST CANCER PATIENTS:ORIGINAL ARTICLE

Shazreen, S, Shahrun Niza, A.S, Cheah Y.K, Richard MC Yu, Fathinul Fikri, A.S

Abstract


Aim: 18F- Fluorocholine uptake increases during malignant transformation and potentially identify the aggressiveness of cancer cells. However the novelty of the 18F-FCH with expression of  miRNA and CD47 were sought as there were exceedingly scare report on their role in underpinning aggressive breast cancers. We examined  the ability of 18F-FCH uptake with expression of CD47 and miRNA in detecting aggressiveness of breast cancers.

Materials and Methods: Twenty one breast cancer patients with BIRADS 4 or 5 on mammogram criteria were recruited and underwent 18F-FCH PET/CT. Histological results were used as gold standard. The standardized uptake value (SUVmax)  was  analysed to determine  the degree of altered choline metabolism on Positron Emission Tomography. The expression of miRNA were measured using quantitative real-time polymerase chain reaction. After the PCR cycles, melting curve analyses were performed to validate the specific generation of the expected PCR product by looking at the upregulated (miRNA expression) or downregulated (low miRNA expression).While CD47 expression were analyzed by using ELISA and the standard curve were construct using regression analysis.

 Results: This study showed that high SUVmax of 18F-FCH is associated with expression of mirna-21 in lymph node with means of 1.81±2.21g/dl,,p=0.05 and metastasis with means of 3.33±3.61g/dl ,p=0.02,while  mirna-155 in lymph node with means of  1.47±1.99g/dl ,p=0.01. Furthermore there is correlation between high SUVmax with means of 1.28±1.90g/dl and  expression of CD47 with means of 0.85±0.23g/dl in lymph node, p=0.008.

Conclusion: 18F-FCH with expression of miRNA and CD47 are novel predicting markers in underpinning  aggressive breast cancers.


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