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Effectiveness of a Quick Validated Monitoring Method for Glucose Levels in Dog Blood Compared to the Traditional Chemical Method

Claudia Karyna Alarcón Colín, Sara Del Carmen Caballero Chacón, Raquel Lopez Arellano, Luis Ocampo Camberos, Dinorah Vargas Estrada


Background: The use of a rapid glucose measurement test (RT) with a glucometer and commercially available test strips was validated and its diagnostic value was compared with the traditional chemical test (CHT), to have a cheaper tool for prevention and the diagnosis of diseases related to the alteration of this monosaccharide in the veterinary clinic.

Materials and methods: 8 adult dogs were used that were more than one year old and were considered an ideal and clinically healthy weight according to the results of the physical examination and the cabinet tests. Blood samples were obtained to create a triplicate glucose curve for each dog and the glucose was evaluated using the two methods, already mentioned. Previously, in vitro validation of the RT test was performed using a glucose standard and an UV-vis spectrophotometry equipment.

Results: The validity of the RT was corroborated as an efficient, reliable, fast, easy and economical diagnostic tool. The linearity and sensitivity of the test complied (R2 ≥ 0.98). The effectiveness of the method was verified, with an acceptance criterion of (80-120%) and a coefficient of variation ≤ 3%.

Conclusion: According to the results obtained, the glucometer is a quick, economical and reliable tool to monitor the blood glucose levels of patients who come to the clinic veterinary for the prevention and / or diagnosis of related diseases such as overweight, obesity, insulin resistance and diabetes, diseases that in the last decades have become very important due to their epidemiological importance in the world.

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