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Immunization of Mice with Recombinant HCV core+E1/E2 Proteins and Montanide

Masoud Ghorbani

Abstract


Background: Current treatments against hepatitis C virus (HCV) infections have limited efficacy and there is no commercially available vaccine. We examined herewith the immune response to HCV core and E1/E2 immunogens.

Results: Balb/c mice were divided into 2 groups of 10 and immunized three times with either Montanide alone or a combination of HCV Core+E1/E2 proteins and montanide. Four weeks after the last immunization, serum samples were collected from both groups for measuring the levels of specific HCV antibodies. Immunization of mice with recombinant core protein and E1/E2 polyprotein and montanide as adjuvant showed a significant increase in the total IgG whereas IgG1 was predominant as determined by ELISA.

Conclusions: We could not detect any IgG2a in the immunized group. The combination of recombinant HCV polyprotein and montanide induced a high antibody titer with a predominance of IgG1 antibodies that recognized the major neutralization epitopes in Hypervariable Region 1 (HVR1).


Keywords


hepatitis C virus, Immunization, Core protein, E1 protein, E2 protein

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