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Detection and Quantification of the IcaA Gene in Staphylococcus aureus during Logarithmic Growth and Stationary Phases

Lotfi Ghellai, Walter Zingg, Adriana Renzoni, Hafida Hassaine


Staphylococcus aureus is a major human pathogen with the ability of biofilm formation. The role of the intercellular adhesion (ica) gene locus in biofilm formation has been widely discussed, but few comparative studies have attempted to evaluate its expression during different growth states. This study evaluated the expression of icaA gene during the logarithmic (log) growth phase and stationary phase of four planctonic biofilmforming S. aureus clinical isolates, which were identified by using matrix-assisted laser desorption ionization time of flight mass spectrometry. The IcaA gene was present in the four isolates in both phases, but with more expression during the logarithmic growth phase compared to stationary phase. During the logarithmic phase, icaA gene expression varied compared to the control strain Sa113 ATCC35556, a well-defined icaA-expressing and biofilm-producing S. aureus. Most clinical strains showed lower expression (by 22-87%), but one strain showed even bypassed the control strain by 100%. 

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