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Isolation and Characterization of Hydrocarbon-Degrading Brevundimonas diminuta DB-19 from Crude Oil-Polluted Seawater

Deng Mao Cheng, Hong Yue Hui, Li Jing, Zhou Qian-Zhi, Chen Wei-Xin, Yuan Jian-Ping, Peng Juan, Wang Jiang-Hai

Abstract


Petroleum pollution in environments has caused an increasingly serious international concern. Among the existing techniques for the treatment of crude oil pollution, microbial remediation has become one of the most reliable strategies for the thorough elimination of petroleum contaminants. Screening bacteria with hydrocarbon-degrading ability is of great significance for the bioremediation. In this study, strain DB-19 with an effective hydrocarbon-degrading ability was isolated from the crude oil-polluted seawater at the Maoming Harbor, southern China, using a screening medium containing diesel oil. Strain DB-19 was identified as Brevundimonas diminuta on the basis of morphological observations, biochemical characterization, and phylogenetic analysis of 16S rDNA sequences. An optimized biodegradation condition of diesel oil by strain DB-19 was determined as follows: temperature, 28 °C; pH value, 7.5; NaCl concentration, 20–30 g/L; rotary speed, 200–250 rpm; and with yeast extract or urea as the sole source of nitrogen. Under these conditions, the total degradation rate of n-alkanes, determined by using a gas chromatography–mass spectrometer (GC–MS), reached up to 93.1% after 10 days of degradation. Strain DB-19 could adapt to a wide range of salinity, and the degradation rate was still higher than 70% under the condition of 40 g/L NaCl. In addition, strain DB-19 could degrade anthracene, phenanthrene, and pyrene after 30 days of incubation, with the degradation rates of 39.5%, 63.1%, and 42.7%, respectively. Thus, strain DB-19 exhibits a good potential for bioremediation. This work contributes to develop a cost-effective and eco-friendly method to remediate crude oil-contaminated marine environments.


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