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Essential oil from Mentha rotundifolia harvested in Northeast Algeria: Chemical Composition, Larvicidal and Enzymatic Activities on Culex pipiens larvae

Rim Kharoubi, Nassima Rehimi, Noureddine Soltani

Abstract


Background: Plant extracts constitute an alternative to conventional insecticides. Culex pipiens is the most abundant mosquito species in Algeria. This research aimed to investigate the eficacy of essential oils from Mentha rotundifolia against the fourth instar larvae of Cx pipeins.

Materials and Methods: The aerial parts of the plant were harvested in the Annaba region (Northeast of Algeria) between March and June 2017, the essential oils EOs) were extracted by a Clevenger hydrodistiller andanalyzed by gas chromatography-mass spectrometry (GC-MS). The larvicidal activities of EOs were tested against fourth instar larvae of Culex pipiens following the procedure of the World Health Organization under laboratory conditions. Furthermore, the effect of EO was examined on the activity of glutathione S-transferase (GST) and acetylcholinesterase (AChE), biomarkers of oxidative stress and neurotoxicity, respectively.

Results: GC-MS analyses revealed sixteen compounds in M. rotundifolia EO and the main components are: Carvacrol (60.54%), Pulegone (5.95%) and M-cymene (5.25%). The larvicidal assay showed that the EO exhibited insecticidal activity against the fourth instar larvae of C. pipiens (LC50= 62.08 ppm and LC90= 178.64 ppm at 72 hour exposure).When applied at it’s LC50 and LC90 EOwas found to present a neurotoxic activity and a stimulation of the detoxification system, as evidenced by an inhibition of AChE and an induction of GST, respectively.

Conclusion: M. rotundifolia EO presents an efficacy against Cx  pipiens larvae and could be considered as a potential alternative to conventional insecticides for mosquito control.


Keywords


Biomarkers, Culex pipiens, Essential Oil, GC-MS, Larvicidal activity, Mentha rotundifolia.

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References


Abbott, W.B., 1925. A method for computing the effectiveness of an insecticide.Journal of Economic Entomology.18, 265–267.

Abdelgaleil, S.A.M., Mohamed, M.I.E., Badawy, M.E.I., El-arami, S.A.A., 2009. Fumigant and contact toxicities of monoterpenes to Sitophilus oryzae (L).and Tribolium castaneum (Herbst) and their inhibitory effects on acetylcholine esterase activity. Journal of Chemical Ecology. 35, 518–525.

Alouani, A., Rehimi, N., Soltani, N., 2009. Larvicidal activity of a Neem tree extract (Azadirachtin) against mosquito larvae in the republic of Algeria. Jordan Journal of Biological Sciences. 2, 15-22.

Alouani, A., Rehimi, N., Soltani, N., 2013. Bioefficacy of azadirachtin in controlling Culex pipiens pipiens (Diptera: Culicidae). Jordan Journal of Biological Sciences. 6, 217-222.

Alouani, A., Rehimi, N., Soltani, N., 2019. Ovicidal Activity of Azadirachtin and Bacillus thuringiensisagainst Eggs of Mosquito Culex pipiens.Mintage Journal of Pharmaceutical & Medical Sciences. 7, 8–12.

Al-Sarar, A.S., 2014. Chemical Composition and Larvicidal Activity of Essential Oils Isolated from Three Saudi Medicinal Plant Species. Bothalia Journal. 44, 170–178.

Benelli, G., 2015. Research in mosquito control: current challenges for a brighter future. Parasitolgy Research. 114, 2801–2805.

Benelli, G., Pavela, R., Petrelli, R., Nzekoue, F.K., Cappellacci, L., Lupidi, G., Quassinti, L., Bramucci, M., Sut, S., Dall’Acqua, S., Canale, A., Maggi, F., 2019. Carlina oxide from Carlinaacaulis root essential oil acts as a potent mosquito larvicide. Industrial Crops and Products. 137, 356–366.

Bohbot, J.D., Durant, N.F., Vinyard, B.T., Dickens J.C., 2013. Functional development of the octenol response in Aedesaegypti.Frontiers in Physiology. 4, 1–8.

Boudjelida, H., Bouaziz, A., Soin, T., Smagghe, G., Soltani, N., 2005.Effects of ecdysone agonist halofenozide against Culex pipiens. Pesticide Biochemistry and Physiology. 83, 115–123.

Bouguerra, N., Tine-Djebbar, F.,Soltani, N., 2018. Effect of Thymus vulgaris L. (Lamiales: Lamiaceae) Essential Oil on Energy Reserves and Biomarkers in Culex pipiens L. (Diptera: Culicidae) from Tebessa (Algeria). Journal of Essential Oil Bearing Plants, 21:4, 1082 1095.

Bouguerra, N., Tine-Djebar, F., Soltani, N., 2019.Oregano Essential Oil as Potential MosquitoLarvicides. Transylvanian Review. 39, 9612–9619.

Brada, M., Bezzina, M., Marlier, M., Carlier, A., Lognay, G., 2007.Variabilité de la composition chimique des HuilesEssentielles de Mentha rotundifolia du Nord de l’Algérie. Biotechnologie, Agronomie, Société et Environnement. 11, 3–7.

Bradford, M.M., 1976.A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry. 72, 248–254.

Clark, A.G., 1989. The comparative enzymology of GST from non-vertebrate organisms. Comparative Biochemistry and Physiology.92, 419–446.

Dris, D., Tine-Djebbar, F., Soltani, N., 2017 a. Lavanduladentata essential oils: chemical composition and larvicidal activity against Culisetalongiareolata and Culex pipiens (Diptera: Culicidae). African Entomology. 25, 387–394.

Dris, D., Tine-Djebbar, F., Bouabida, H., Soltani, N., 2017 b. Chemical composition and activity of an Ocimum basilicum essential oil on Culex pipiens larvae: Toxicological, biometrical and biochemical aspects. South African Journal of Botany. 113, 362–369.

El Arch, M., Satrani, B., Farah, A., Bennani, L., Briky, D., Fechtel, M., Blaghen, M., Talbi, M., 2003 .Chemical composition and antimicrobial and insecticidal activities of the essential oil in Mentha rotundifolia from Morocco. ActaBotanicaGallica. 150, 267–274.

Ellman, G.L., Courtney, K.D., Andres, V., Featherstone, R.M., 1961. A new and rapid colorimetric determination of acetylcholinesterase activity. Biochemical Pharmacology. 7, 88–95.

Finney D.J., 1971. Probit Analysis,third ed. Cambridge University Press, London and New York.

Fisher, R.A., Yates, F., 1957.Statistical tables for biological agricultural and medical research, fifth ed.Olivirer& Boyd, London.

Giray, E.S., Kirici, S., Kayab, D.A., Türk, M., Sönmez, Ö., Inan, M., 2008. Comparing the effect of sub-critical water extraction with conventional extraction methods on the chemical composition of Lavandula stoechas. Talanta. 74, 930–935.

Guenez, R., Tine-Djebbar, F., Tine, S.,Soltani, N., 2018. Larvicidal Efficacy of Mentha pulegium Essential Oil against Culex pipiens L. and Aedes caspius P. larvae. World Journal of Environmental Sciences. 7, 1–7.

Habig, W.H., Pabst, M.J., Jakoby, W.B., 1974. Glutathione S-transferases. The first enzymatic step in mercapturic acid formation. Journal of Biological Chemistry. 249, 7130–7139.

Hamaidia, K., Soltani, N., 2014. Laboratory evaluation of a biorational insecticide, kinoprene, against Culex pipiens larvae: effects on growth and development. Annual Research and Review in Biology. 4, 2263–2273

Hamaidia, K., Soltani, N., 2016. Ovicidal activity of an insect growth disruptor (methoxyfenozide) against Culex pipiens L. and delayed effect on development.Journal of Entomology and Zoology Studies. 4, 1202–1207.

Hamaidia, K., Tine-Djebbar, F., Soltani, N., 2018. Activity of a selective insecticide (methoxyfenozide) against two mosquito species (Culex pipiens and Culiseta longiareolata): toxicological, biometrical and biochemical study. Physiological Entomology. 43, 315–323.

Hamaidia, K., Soltani, N., 2019. Compensation of kinoprene effect on reproduction of Culex pipiens by methoxyfenozide, an ecdysone agonist. Journal of Entomological Research. 43 (2), 125–130.

Isman, M.B., Miresmailli, S. Machial, C., 2011. Commercial opportunities for pesticides based on plant essential oils in agriculture, industry and consumer products. Phytochemistry Reviews. 10, 197–204.

Jakoby, W.B., Habig, W.H., (1980). Glutathion transferase. In: Jacoby, W.B (Eds). Enzymatic basis of detoxification. Academic press. New York. 2, 63–94.

Jennings, W., Shibamoto, T., 1980. Qualitative analysis of flavour and fragrance volatile by glass capillary gas chromatography. New York: Academic Press.

Koliopoulos, G., Pitarokili, D., Kioulos, E., Michaelakis, A.,Tzakou, O., 2010. Chemical composition and larvicidal evaluation of Mentha Salvia and Melissa essential oils against the West Nile virus mosquito Culex pipiens. Parasitology Research. 107, 327–335.

Kokkini, S., Papageorgiou, V.P.,1988. Constituents of Essential Oils from Mentha x rotundifolia Growing Wild in Greece. Planta Medica. 38, 166–167.

Kumar, P., Mishra, S., Malik, A.,Satya, S., 2011. Insecticidal properties of Mentha species: A review. Industrial Crops and Products. 34, 802–817.

López, M.D., Pascual-Villalobos, M.J., 2010. Mode of inhibition of acetylcholinesterase by monoterpenoids and implications for pest control. Industrial Crops and Products. 3, 284–288.

Lorenzo, D., Paz, D., Dellacassa, E., Davies, P., Vila, R., Cañigueral, S., 2002. Essential Oils of Mentha pulegium and Mentha rotundifolia from Uruguay.Brazilian Archives of Biology and Technology. 45, 519–524.

Manzano-Román, R., Oleaga, A., Pérez-Sánchez, R., Siles-Lucas, M., 2012. Gene Silencing in Parasites: Current Status and Future Prospects. Advances in Parasitology. 78, 1–55.

Pandey, S.K., Upadhyay, S., Tripathi, A.K., 2009. Insecticidal and repellent activities of thymol from the essential oil of Trachys permumammi (Linn) Sprague seeds against Anopheles stephensi. Parasitology Research. 105, 507–512.

Pavela, R., 2014.Insecticidal properties of Pimpinella anisum essential oils against the Culex quinquefasciatus and the non-target organism Daphnia magna. Journal of Asia-Pacific Entomology.17, 287–293.

Pavela, R., 2015. Essential oils for the development of eco-friendly mosquito larvicide: A review. Industrial Crops and Products, 76: 174–187.

Pavela, R., Zabka, M., Bednar, J., Tˇríska, J.,Vrchotová, N.,2016. New knowledge for yield, composition and insecticidal activity of essential oils obtained from the aerial parts or seeds of fennel (FoeniculumvulgareMill.). Industrial Crops and Products.83, 275–282.

Pérez Raya, M.D., Utrilla, M.P., Navarro, M.C., Jiménez, J., 1990. CNS activity of Mentha rotundifolia and Mentha longifolia essential oil in mice and rats. Phytotherapy Research. 4, 232–234.

Pino, J.A., Rosado, A., Fuentes, V., 1999. Chemical composition of the leaf oil of Mentha rotundifolia (L.) Hudson from Cuba.Journal of Essential Oil Research. 11, 241–242.

Rehimi, N.,Soltani, N., 1999. Laboratory evaluation of Alsystin, a chitin synthesis inhibitor, against Culex pipiens L. (Diptera: Culicidae): effects on development and cuticule secretion. Journal of Applied Entomology. 123, 437–441.

Rehimi, N.,Soltani, N., 2002. Laboratory evaluation of Andalin, an insect growth regulator interfering with cuticle deposition, against mosquito larvae. Sciences &Technologie.18, 106–110.

Rehimi, N., Alouani, A., Soltani, N., 2011. Efficacy of Azadirachtin against Mosquito Larvae Culex pipiens (Diptera: Culicidae) under Laboratory Conditions.European Journal of Scientific Research. 57, 223–229.

Riahi, L., Chograni, H., Elferchichi, M., Zaouali, Y., Zoghlami, N., Mliki, A., 2013. Variations in Tunisian wormwood Essential Oil profiles and phenolic contents between leaves and flowers and their effects on antioxidant activities. Industrial Crops and Products. 46, 290–296.

Rocha, D., Novo, M., Matos, O., Figueiredo, A.C., Delgado, M., Cabral, M.D., Liberato, M.,Moiteiro C., 2015.Potential of Mentha pulegium for mosquito control.Revista de CiênciasAgrárias.38, 155–165.

Seghier, H., Tine-Djebbar, F., Loucif-Ayad, W., Soltani, N., 2020.Lavicidal and Pupicidal Activities of Petroselinum crispum Seed Essential Oil on Culex pipiens and Culiseta longiareolata Mosquitoes. Transylvanian Review. 47, 14669–14677.

Skoog, D.A., Holler F.J., Nieman, T.A., 2003. Principles of instrumental analysis, First ed. Ed. De Boeck University, Bruxelles.

Soltani, N., Rehimi, N., Drardja, H., Bendali. F., 1999. Activité du triflumuron à l'égard de Culex pipiens et impacts sur deux espèces larvivores non visées. Annales de la Société Entomologique de France. 35, 59–64.

Swaroop, S., Gilroy, A.B.,Umera, K., 1966. Statistical methods in Malaria eradication. World Health Organisation. Geneva.

Tine-Djebbar, F., Soltani, N., 2008.Activité biologique d’un agoniste non stéroïdien de l’hormone de muesur Culiseta longiareolata: analyses morphométrique, biochimique et énergétique. Synthèse. 18, 23–34.

Tripathi, A.K., Prajapati, V., Ahmad, A., Aggarwal, K.K., Khanuja, S.P.S., 2004. Piperitenone oxide as Toxic, Reppellent and Reproduction Retardant toward Malarial Vector Anopheles stephensi (Diptera: Anophelinae).Journal of Medical Entomology. 41, 691–698.

World Health Organization., 2005. Guidelines for Laboratory and Field Testing of Mosquito Larvicides. Communicable Disease Control, nPrevention and Eradication, WHO Pesticide Evaluation Scheme. WHO, Geneva (WHO/CDS/WHOPES/GCDPP/1.3).


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